Microbiology / Molecular Biology / Virology

Under the expert supervision of  medical microbiologists, assisted by experienced and skilled medical laboratory technologists, the Department of Microbiology and Molecular Biology of Islamabad Diagnostic Centre offers State-of-the-Art diagnostic solutions for major infectious disease problems faced by the health care providers in this region.

Culture & Anti-Microbial Drug Susceptibility 
Besides the routine culture and sensitivity of common pathogens involved in common infections, both hospital acquired as well as community acquired, IDC offers a comprehensive high-tech diagnostic service for Viral Hepatitis (Hepatitis A, B, C and E), Tuberculosis, Salmonellosis (Typhoid), Water-borne Diseases, Sexually Transmitted Infections including HIV/AIDS and common Fungal infections.

In spite of tremendous advances in medical science, precise and early diagnosis of tuberculosis continues to be a major clinical and public health problem in our health care set-up.
IDC has accepted the challenge of providing a rapid, reliable and cost effective diagnosis of both active and latent tuberculosis.

The current range of TB diagnostics at IDC includes:

  1. AFB Smear Microscopy
  2. Tuberculin Skin Test (TST – Mantoux test)
  3. AFB Culture – using BACTEC MGIT 960 Culture System (Becton Dickinson)
  4. Interferon Gamma Release Assay – (IGRA) namely T.SPOT-TB
  5. Nucleic acid Amplification Tests (NATs) by PCR (GenoType MTBDRplus )
    which incorporates drug susceptibility against first line  anti-tubercular drugs
  6. Rapid (Immunochromatographic – ICT) assays
  7. Enzyme-linked Immunosorbent Assays (ELISA)

Blood culture is performed on BACTECTM 9050 – a unique high performance blood culture system based on fluorescent sensor technology which ensures shorter reporting time – average 12 hours for preliminary report, coupled with highest recovery of pathogens (>85%) and 25% more positives on day 1, along with optimal neutralization of antibiotics in blood culture sample even if the patient is taking antibiotics.

Besides, IDC is probably one of the very few laboratories in Pakistan which offers antifungal drug susceptibility testing against Candidainfections.

Quality Assurance: Authenticity of microbiological investigations at IDC is ensured by a strict internal quality assurance program which is supplemented by regular participation in the National External Quality Assurance Program (NEQAAP) being run countrywide by the Armed Forces Institute of Pathology Rawalpindi.

IDC Virology:

  1. Viral Hepatitis

For the initial diagnosis of viral hepatitis, IDC offers highly sensitive and specific assays (Abbott MEIA) for all the major types of viral hepatitis including Hepatitis A (IgG and IgM), Hepatitis B complete profile (HBsAg and Ab, anti-HBc total and IgM, HBeAg and Ab), Hepatitis C Ab and  the water- born Hepatitis E (IgG and IgM).
Hepatitis B and C, the most serious forms of viral hepatitis, not only affect millions of people in our country but have also the tendency to become chronic, leading to serious long term sequelae including cirrhosis and hepatocellular carcinoma. Since both are now virtually treatable in a vast majority of patients, both require molecular diagnosis by PCR, qualitative as well as quantitative to assess the viral load (HBV-DNA and HCV- RNA). With the recent introduction of highly effective oral therapy using Directly Acting Antivirals (DAAs), genotyping, particularly of HCV, has assumed crucial importance. IDC feels privileged to offer one of the best Real Time PCR services for molecular diagnosis of HBV and HCV:-

  • Qualitative PCR (to detect HBV –DNA or HCV-RNA)
  • Quantitative PCR (Viral Load of HBV-DNA or HCV-RNA)
  • Genotyping (both HBV & HCV)

Quality Assurance: Authenticity of Virological investigations at IDC is ensured by a strict internal quality assurance program which is supplemented by regular participation in the Randox International Quality Assessment Scheme (RIQAS) and College of American Pathologist (CAP) Proficiency Testing.

  1. Other Viral Infections

IDC offers reliable diagnostic services for the infections including HIV/AIDS, Rubella, Herpes simplex (HSV), Cytomegalovirus (CMV) and Dengue etc.

For an appointment and to get this service

Please call Patient Services Department at
UAN # : 051-111-000-432
Disclaimer: Every diagnostic test has scientific acceptable technology or technique based limitations of uncertainty of measurement, false positive or false negative and so do not fall under the domain of negligence. In case of any such scenario, we offer free repeat of test within 24-48 hours.